The NADH decrease is directly proportional to the ALT activity.(Package insert: ALT reagent. Roche Diagnostics 04/2019)Īlanine aminotransferase (ALT) activity is determined by a kinetic method using a coupled enzyme reaction where the rate of NADH consumption is measured at 340 nm. It is determined by measuring the increase in absorbance.(Package insert: Alkaline Phosphatase Gen.2 reagent. The p-nitrophenol released is directly proportional to the catalytic alkaline phosphatase activity. In the presence of magnesium and zinc ions, p-nitrophenyl phosphate is cleaved by phosphatases into phosphate and p-nitrophenol. Roche Diagnostics 02/2022)Īspartate aminotransferase (AST) is measured by a coupled enzyme kinetic method where the rate of decrease of NADH, determined at 340 nm, is directly proportional to the AST activity.(Package insert: AST reagent. The color intensity of the blue-green albumin-BCG complex is directly proportional to the albumin concentration and is determined photometrically.(Package insert: Albumin Gen.2 reagent. The dye, bromcresol green (BCG), is added to serum in an acid buffer. The color intensity is directly proportional to the protein concentration, which can be determined photometrically.(Package insert: Total Protein Gen.2 reagent, Roche Diagnostics 11/2019) Sodium potassium tartrate prevents the precipitation of copper hydroxide and potassium iodide prevents autoreduction of copper. Roche Diagnostics 11/2021)ĭivalent copper reacts in alkaline solution with protein peptide bonds to form the characteristic purple-colored biuret complex. The rate of NADPH formation is directly proportional to glucose concentration and is measured photometrically.(Package insert: Glucose HK Gen.3 Reagent. In the presence of nicotinamide adenine dinucleotide phosphate (NADP+), glucose-6-phosphate dehydrogenase oxides G6P to gluconate-6-phosphate and NADPH. Glucose in the serum, in the presence of hexokinase, is converted to glucose-6-phosphate (G6P). The change in absorbance is directly proportional to the calcium concentration and is measured photometrically.(Package insert: Calcium Gen.2 reagent. This complex reacts in the second step with EDTA. Roche Diagnostics 02/2019)Ĭalcium ions react with NM-BAPTA (5-nitro-5'-methyl-1,2-bisethane-N,N,N',N'-tetraacetic acid) under alkaline conditions to form a complex. The color intensity of the quinone imine chromogen formed is directly proportional to the creatinine concentration in the reaction mixture.(Package insert: Creatinine. Catalyzed by peroxidase the liberated hydrogen peroxide reacts with 4-aminophenazone and HTIB (hydroxyiodobenzene) to form a quinone imine chromogen. This enzymatic method is based on the conversion of creatinine with the aid of creatininase, creatinase, and sarcosine oxidase to glycine, formaldehyde, and hydrogen peroxide. The decrease in absorbance, due to the consumption of NADH, is measured kinetically and is proportional to the amount of urea in the sample.(Package insert: Urea/BUN reagent. The ammonia formed then reacts with alpha-ketoglutarate and NADH in the presence of urease/glutamate dehydrogenase to yield glutamate and NAD+. This is a kinetic UV assay where urease cleaves urea to form ammonia and carbon dioxide. The following equation is used to calculate the anion gap (A gap): ![]() The rate of change is directly proportional to the concentration of bicarbonate.(Package insert: Bicarbonate reagent Roche Diagnostics 04/2019) The consumption of NADH causes a decrease in absorbance and is monitored in the UV range of 320 nm to 400 nm. The oxaloacetate produced is coupled with NADH in the presence of malate dehydrogenase (MDH) to produce malate and NAD. Bicarbonate (HCO3-) reacts with phosphoenolpyruvate (PEP) in the presence of phosphoenolpyruvate carboxylase (PEPC) to produce oxaloacetate and phosphate. An electronic calculation circuit converts EMF of the sample to the ion concentration of the sample.(Package insert: ISE Indirect Na, K, CL for Gen2. ![]() The EMF of the reference electrode is constant. The ion selective electron (ISE) module indirectly measures the electromotive force (EMF) difference between an ISE and a reference electrode. The EMF of the ion-selective electrode is dependent on the ion concentration of the sample. Ion-selective electrode (ISE) (indirect potentiometry). ![]() ![]() Reference values have not been established for patients who are younger than 12 months of age.
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During high school and college, she dyed her hair, starting with 2 red streaks (similar to Boyinaband except with 1 red streak), and then fading colors at the end of the hair. Older videos shown in Jaiden's videos show her hair being long and straight in childhood. Her third question was on Comic Villains, and she answered "The Penguin", but Ellen challenged, and Jaiden lost her last bar of life, eliminating her from the game. After that, her second question was on Planets, which she answered "Mercury", but Hank (the green player) challenged, and she lost her first life, then she and Ellen (the blue player) didn't challenge Hank on "decimetre", dropping them both to their last life. Her first question was on Colorful Birds, which she answered "peacock", and she got her question right. During the episode, she played in the first round as the Yellow player. The only other time Jaiden has shown her face on a different YouTuber's channel (that is known so far) is on Tom Scott's channel during the first episode of his series, "Disconnected". Jaiden never revealed her face officially until April 6, 2017. In a later comment by Rebecca, Jaiden said that she was fine with her face being shown on her 2016 VidCon video. In the VidCon 2016 video from Rebecca Parham (also known as Let Me Explain Studios), she showed a picture with herself and Jaiden. However, later on, Jaiden let them upload the original video. She was mortified and forced herself to explain why she didn't want people to see her. However, during the convention, her face was leaked by an undisclosed person. During VidCon 2016, Jaiden also said she took pictures with people, but she wished she didn't. Jaiden initially did not reveal her face because she didn't want people to have an opinion on her based on her looks. In her announcement videos, Jaiden is depicted as a "gremlin", being completely colorless, except having a purple outline. Jaiden has dot eyes and multiple ear piercings, Her outfit doesn’t have any color (though she wears lavender, as depicted in her profile). This initially created a large divide in her fandom, but the reception for her content gradually swang back into the direction of mostly praise. It is at this same time that she revealed that she was in a relationship with a then at the time 9 year old boy and stated to make story time videos about her previous pedophilic incounters as well as record herself making love to her underaged lover. In November 2019, she started to take part in pedophile pride parades, shocking many fans. She was an individual YouTuber, but later on in 2018, she built up a team of animators working for her, so she can chill out. Jaiden later uploaded longer story-time animations, in a tight schedule of 2 weeks. The channel initially started out with short animations and speed art (though most speed art videos were likely later set to private), as well as piano videos. Jaiden first created her channel on February 16, 2014. The channel noticed her artwork, and she was offered to do an animation in the channel, and so, she started to make animations on iHasCupquake's channels. That would be sssir8 and thrumbo.Her career started when Jaiden sent a fanart to iHasCupquake, who Jaiden watched and liked at the time. Who are the artists for the avatar and the banner and the image in the sidebar? No it’s not, only anthropomorphic furry characters, only human (youtuber avatar) x furry stuff unless it’s a specific instance that’s allowed by a mod. Is porn of say dogs or cats or furries in general allowed? We aren’t on good terms with all of the members but we are friendly with the leaders of the these groups. Ask for one if given permission, the majority the time mods will give that out when we want to only.Īre you guys cool with the other jaiden animations discord servers and subreddits? very obviously yes you must be of legal age in America to view this type of content or be in our server and sub all children need to leave. Definitely not we hold ZERO disrespect for any type of content creator that is lewded here this is purely for enjoyment. Yes we do associate with them they are out partners and friends.ĭo you guys hate jaiden or any other animators? No we don’t and we most likely never will.ĭo you associate with the animators that make this amazing rule34 content? literally nobody but the mods of that sub know what happened it could have been privatized or banned for literally an infinite number of reasons. The owner of that server was to but lightly, a huge jerk who we don’t associate with anymore. He left due to personal reasons and transferred ownership to u/wreckitbusmaster99 first guy was a jerk who left because he is a moron. ![]() What happened to the old owners of the subreddit and discord? ![]() This number was originally done by Frankie Miller, a semi-successful British blues-folk singer-songwriter.
Popular posts include Snapchats of women performing blow jobs allegedly sent to boyfriends by the person receiving the blowjob, or screenshots between a woman and a guy she’s excited to sleep with, sent to her husband to make him jealous. Still, members of the group seem to have little preference either way, so long as it looks like an honest text message exchange. The tags are meant to separate fantasy from reality, but the posts are nearly indistinguishable - plenty of those labeled as real could be from porn, or otherwise fabricated for the sake of sharing them on the subreddit. It’s unclear whether everyone involved in the “real” text messages consents to being shared on the subreddit, and there’s no rule asking posters to prove consent, either. ![]() Those tagged “fantasy” are intended to be understood as fictional, while untagged posts are meant to be interpreted as real. ![]() There are six categories of posts in the sub: Cheating, hotwife, cuckold and “fantasy” versions of all three. It’s the context of a sex act that matters most here, not just the actual act itself. Devoted exclusively to screenshots of text messages between a cuck and his hotwife - or between a hotwife and her bull - the subreddit mostly offers verbal content, but the texts often include nudes or Snapchat images, too. R/HotWifeTexts, which has more than 161,000 members, is one of the main venues for this type of erotica (though cuck texts can also be found on other hotwife/cuckold subreddits, old Tumblr accounts like TextMessageMan, Imgur and Pornhub). The fetish here is twofold - it’s both the topic of conversation and the voyeuristic thrill of peering into someone else’s relationship.Īnother set of texts for you guys from hotwifetexts But rather than simply sharing porn on the topic, members specifically want to see the text messages that shape these dynamics as they unfold in real life. On subreddits like r/hotwifetexts, people post and seek screenshots that document cuck relationships like these. While you wait, you giddily share this perfect example of your hotwife relationship to Reddit, where thousands of people will be soon served with an intimate snapshot of this part of your life. Soon after receiving the video, she texts you about how she can’t wait to get home for you to get your turn. ![]() Are you angry? Of course not - if you switch over to your messages with your wife, there’s a long exchange between the two of you where she talks about meeting a guy at a bar, with you encouraging her to go to his place. In the message is a video of your beloved wife riding a mystery man, shot POV-style. You open your phone to see a text from an unknown number. ![]() Overall, VSDC Free Audio Converter is great for converting M4A to MP3 for free. If needed, you can adjust the output quality, frequency, and bitrate. You can even open and convert M3U files.īesides, this free audio converter also offers a tag editor for modifying the title, author, album, genre, etc., as well as a built-in media player. More importantly, all popular audio formats are supported. It has a tabbed interface that’s easy to understand and operate and provides you with the convenience of converting local audio files and online ones. VSDC Free Audio Converter is another best free M4A to MP3 converter that be compatible with all Windows operating systems.
In 2007, Williams provided the voice of A Pimp Named Slickback in The Boondocks. He next appeared as a regular on Wild 'n Out on MTV during its first three seasons. He appeared in the official music video for Nick Cannon's single " Gigolo" in 2003. Williams is best-known for his character Money Mike in the film Friday After Next (2002), and has played supporting characters in Norbit (2007) and First Sunday (2008). In 2002, Williams made his acting debut on NYPD Blue on the sixth episode of its tenth season on October 29. In 2022, he released a new stand-up special on Netflix called World War III. In 2018, Williams released a new stand-up special on Netflix called Great America and shot in Jacksonville, Florida. This is the open discussion that we've had since 2003. Part of my guarantee in my ticket price is that I'm going to be talking about what we are talking about now, and discussing from now to the next time we see again. I am one of the rare urban public officials. This is probably going to go down as one of my finest works, just because it's a collection of forbidden topics that we can't seem to get answered. We all, at some point, if we're are at a certain age, we grew up thinking Pluto was a planet. That's the basis for all conspiracy theories: the fact that there is hidden information out there, and how our process changes about things that we thought we used to know. We know that there are conspiracies out there, but this is a conversation that encompasses a lot of things that aren't being discussed other places. In September 2015, during an interview, Williams announced and described his upcoming Conspiracy Theory Tour: "The conspiracy conversation is a conversation that we are all familiar with. On August 16, 2014, he returned with a new HBO special titled Katt Williams: Priceless: Afterlife, which was directed by Spike Lee. ĭuring late 2013, Williams was on his Growth Spurt Tour. Williams' November 16 performance at the Oracle Arena in Oakland ended after he engaged in a profanity-laced confrontation with a heckler, and was assisted off stage by his own security. A November 1 performance at the Wells Fargo Theatre in Denver ended after he jumped off the stage to confront a heckler. Two live performances in November 2012 ended early because of Williams' confrontational behavior. However, three days later, he announced he would not retire. ![]() Later that year, one day after an incident at a Seattle bar - in which he reportedly acted threatening and violent, then resisted arrest - landed him in jail, he announced the end of his stand-up comedy career. Īfter a four-year hiatus, Williams returned to stand-up in 2012 for his third HBO comedy special, Kattpacalypse. Keeping busy releasing comedy DVDs and touring, Williams had a comedy tour that was named the best of 2008 by Billboard. In 2008, he released his second HBO comedy special, It's Pimpin' Pimpin'. The film had critical success and established Williams as a mainstream comedian. In 2007, he co-wrote and starred as himself in the comedy film Katt Williams: American Hustle. His first HBO stand-up special came in 2006 with The Pimp Chronicles, Pt. Williams starred in his first comedy special, Let a Playa Play, in 2006. Most notably, he appeared on BET's ComicView as Katt "N da Hatt" Williams. ![]() He honed his comic delivery by performing his routine in clubs around the country and had become an established comic by 1999, appearing at the likes of The Improv, The Comedy Club, The Ice House, and Hollywood Park Casino. Williams started performing comedy in the Avondale neighborhood of Cincinnati. ![]() He stated that he wanted to remain God's friend. Though homeless and living in a park, he supported himself as a street vendor. Williams emancipated himself from his parents at age 13 and moved to Florida. ![]() During his childhood, he lived in Haiti for a year and a half on religious mission trips with his family. He stated that he learned to read at three years old, and was communicating in multiple languages, including French and Creole. He was raised in Dayton, Ohio by Jehovah's Witness parents. Micah Williams was born in Cincinnati, Ohio, on September 2, 1971. In 2008, he voiced himself in the video game Grand Theft Auto IV. He played Money Mike in Friday After Next, was a recurring guest on Wild 'n Out, portrayed Bobby Shaw in My Wife and Kids, provided the voice of A Pimp Named Slickback in The Boondocks and Seamus in Cats & Dogs: The Revenge of Kitty Galore, and portrayed Lord Have Mercy in Norbit. Micah " Katt" Williams (born September 2, 1971) is an American stand-up comedian and actor. Black comedy, blue comedy, satire, observational comedy, physical comedyĪfrican-American culture, racism, politics, celebrities, sex ![]() The organisation has a strong approach to supporting open source software and to data protection and privacy. Mozilla is an international non-profit organisation whose revenues are reinvested in product development. They are globally driven by profit and profitability to satisfy their shareholders. ![]() Google and Microsoft are web giants (GAFAM) that are among the largest American companies dominating the global digital market. Publishers use it for their own needs, to develop new features or to pay their shareholders. Multiplied by the number of Internet users and searches in the world, the sums are considerable. Advertisers are willing to pay search engine publishers to show their ads. But it is complex software, in which thousands of man-hours have been invested and are continuously invested by the publishers, which is not free! The publishers of this software need to finance it an important source of income is available to them: advertising. The use of search engines is free for users: no installation fees, no search fees. ( 16) Lilo uses electricity produced in France, which makes it 96% decarbonised. ( 15) Ecosia is 200% renewable thanks to the solar energy it produces. 27% of energy purchased in 2019 was from renewable sources. ( 14) On another scale, Mozilla has a transparent and inclusive approach to sustainability. ( 13) Similarly, Microsoft has committed to 100 percent renewable energy by 2025, set a goal of 24/7 carbon-free power by 2030, and purchased 7.8 gigawatts of renewable energy projects globally. Since 2017, Google has been using renewable energy to cover its annual electricity consumption, has set a goal of 24/7 carbon-free energy by 2030 for all its operations, and has purchased 5.5 gigawatts of renewable energy in 2019. The giants Google and Microsoft have committed to renewable energy. The sector as a whole is increasingly moving towards renewable energy, with some organisations being more ambitious than others. There is little public benchmarking of the type of energy used by them. It is difficult to compare the carbon intensity of electricity consumed by web browsers and search engines. ![]() Browsers and search engines that block the transfer or do not collect personal data (Firefox, Lilo, Ecosia) contribute to less direct and indirect energy consumption. The more data that circulates, the more energy is consumed. ( 12)įurthermore, every internet browsing or search produces data, which is sometimes transmitted, collected and stored by dozens or even hundreds of companies interested in this information (big data). While the browsers differ little overall in terms of pure navigation, there are significant differences in energy consumption for other functions, with a ratio of more than 3 (Firefox Preview/Focus in the lead). ( 11)Īt the top of the list of the most energy-efficient browsers in 2020 are: Vivaldi, Firefox Preview, DuckDuckGo, Lilo, Kiwi. Search engines that do not automatically display an interactive map or widget are also less energy intensive (Ecosia, StartPage, Lilo). The more additional functionality there is, the more power hungry the system is. In general, a shorter search process has less energy and battery impact and can help reduce the overall carbon impact of the whole chain. A search consumes more energy via a mobile browser than with an application (64% carbon savings on average). The energy consumption of internet searches is based on both network and user energy consumption. Some browsers are also more energy efficient than others. ![]() While traditional web browsers and search engines dominate the global market ( 5), they do not all have the same ecological footprint and more ethical and/or ecological search engines like Ecosia or Lilo are starting to stand out, especially in Europe. ( 4) It is estimated that Google receives 99 000 requests per second. ( 3)Īlmost 93% of Internet traffic comes from search engines. ( 2) By way of comparison, the energy consumption of a data centre is equivalent to that of 30 000 people in Europe. Data centres and data transmission networks are responsible for nearly 1% of energy-related greenhouse gas emissions. The electricity used comes mainly from non-renewable energies that pollute. As for a computer, these installations are very energy consuming. This data is stored in infrastructures: computer centres – or data centres. ( 1) Browsing or searching on the Internet consists in transmitting information materialised by data. Today, digital technology accounts for 3 to 4% of the world’s greenhouse gas emissions, and this share is constantly increasing.
![]() Volume Two: Gut Bucket Blues, Skit-Dat-De-Dat, Two Deuces, Butter and Egg Man, Knee Drops, Heebie Jeebies, Irish Black Bottom, Sunset Cafe Stomp, Come Back Sweet Papa, Oriental Strut. Volume One: Muggles, Struttin’ with the Barbecue, Cornet Chop Suey, Tight Like This, My Monday Date, Jazz Lips, Gully Low Blues, Hotter Than That, I’m Not Rough, Potato Head Blues. ![]() When you are ready, play through the solos, then grab an immediate PDF download above. Be sure to click through them all to get the full list of tunes included. Search Within: Trumpet Improv, Trumpet Play Alongs, Trumpet Solos and Transcriptions, Trumpet Technique. In the samples you will find both book covers as well as the first page from the solo and score of each book. You will get both volumes for one discounted price to get more Louis for less. ![]() Usually this is sold as two books with two full price tags, but I choose to combine them together. These solos are ready for performance with your pianist and/or rhythm section, and some of the most smokin’ tunes of Armstrong’s career. Louis Armstrong’s Twenty Jazz Solos for Trumpet and Piano are transcriptions from actual recordings that include full annotated trumpet parts with chord changes included, as well as a fully notated piano part. This well-known melody begins with a moderate Latin feel around. While we strive to stick to our scheduled timetables, we can’t guarantee arrival or departure times. What happens if the Greyhound bus from Durban to Johannesburg gets delayed or cancelled? For the older kids, 36 months and up, they’ll need an adult ticket. ![]() And if they’re under 36 months, they can ride for free as long as they’re happy to sit on your lap. If your child is under 12 years, they’ll need to have an adult with them. We’re all about making sure everyone has a safe and comfortable journey. Just a note, for the safety and comfort of all our passengers, we might have to say no to those who are unwell or to women in the later stages of pregnancy. For passengers with disabilities or those using wheelchairs, we suggest letting our reservations team know about your situation when you’re securing your ticket. If you have a medical condition or disability, it’s always best to consult with your doctor before hitting the road. What should I do if I have a medical condition or disability? Although it’s important to keep in mind, we can’t always guarantee the availability of onboard entertainment. We strive to keep the ride entertaining with family-friendly videos and music on all our coaches. When you travel with us on our luxury Dreamliners or single deckers, you’re guaranteed a 5-star journey. What kind of facilities can I expect on the bus? Greyhound: Bus tickets from Durban to Johannesburg FAQs 1. Our luxury Dreamliners and single deckers are built to 5-star specifications that ensure your journey from Durban to Johannesburg is an experience defined by reliability, comfort, professionalism and exceptional service. When luxury craves affordability, Greyhound coaches shine the brightest. With high standards and morals, Greyhound continues to be the safe and reliable luxury coach operator of choice to travel from Durban to Johannesburg. Since 1984 Greyhound has brought you South Africa’s favourite luxury coach solution, connecting cities as old as the country itself and towns as brilliantly curious as the cultures that connect us. |
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